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Lipidomik testlerimizde LC-MS/MS tabanlı Orbitrap teknolojisi kullanılmaktadır. HRAM (Yüksek Çözünürlüklü Hassas Kütle) spektrometrisindeki gelişmeler, lipid analizinin zorluklarını aşmış; eşsiz bir duyarlılık, seçicilik ve kesinlik düzeyi sunmaya başlamıştır. Literatür temelli protokoller ve Avanti Splash Lipidomix standartları ile lipid analizine uygun örnek hazırlığı Ölçümlerin Thermo Scientific Orbitrap Exploris 240 MS sistemi ile gerçekleştirilmesi Lipid tanımlama için LipidSearch ve Compound Discoverer yazılımlarının kullanımı Verilerin Compound Discoverer 3.3 yazılımı ile işlenmesi Analiz sonuçları yaklaşık 2 ila 3 hafta içinde teslim edilmektedir.","absoluteBoundingBox":{"x":32466.0,"y":28900.439453125,"width":1152.0,"height":260.0},"isolatedAbsoluteRenderBounds":{"x":32466.341796875,"y":28907.029296875,"width":1122.654296875,"height":251.189453125},"relativeTransform":[[1.0,0.0,0.0],[0.0,1.0,50.0]],"size":{"x":1152.0,"y":260.0},"fills":[{"opacity":0.550000011920929,"blendMode":"NORMAL","type":"SOLID","color":{"r":1.0,"g":1.0,"b":1.0,"a":1.0},"visible":true}],"strokeAlign":"OUTSIDE","layoutAlign":"STRETCH","strokes":[],"effects":[],"accessibleHTMLTag":"AUTO","isDecorativeImage":false,"ariaAttributes":{},"interactions":[],"characterStyleOverrides":[],"characters":"LC-MS teknolojisi, bir karışım içindeki bileşenlerin önce UHPLC (Ultra Yüksek Performanslı Sıvı Kromatografisi) ile ayrıştırılmasına, ardından bu bileşenlerin iyonlaştırılarak kütle/yük (m/z) oranlarına göre birbirinden ayrılmasına dayanır. Lipidomik testlerimizde LC-MS/MS tabanlı Orbitrap teknolojisi kullanılmaktadır. HRAM (Yüksek Çözünürlüklü Hassas Kütle) spektrometrisindeki gelişmeler, lipid analizinin zorluklarını aşmış; eşsiz bir duyarlılık, seçicilik ve kesinlik düzeyi sunmaya başlamıştır.\n\nLiteratür temelli protokoller ve Avanti Splash Lipidomix standartları ile lipid analizine uygun örnek hazırlığı\nÖlçümlerin Thermo Scientific Orbitrap Exploris 240 MS sistemi ile gerçekleştirilmesi\nLipid tanımlama için LipidSearch ve Compound Discoverer yazılımlarının kullanımı\nVerilerin Compound Discoverer 3.3 yazılımı ile işlenmesi\nAnaliz sonuçları yaklaşık 2 ila 3 hafta içinde teslim edilmektedir.","lineIndentations":[0,0,1,1,1,1,1],"lineTypes":["NONE","NONE","UNORDERED","UNORDERED","UNORDERED","UNORDERED","UNORDERED"],"listStartOffsets":[],"lineStyleOverrides":[0,0,0,0,0,0,0],"lineTextDirections":null,"textAutoResize":"HEIGHT","textAlignVertical":"CENTER","style":{"fontFamily":"Outfit","fontPostScriptName":"Outfit-Medium","fontStyle":"Medium","textAutoResize":"HEIGHT","fontVariantPosition":"NORMAL","fontSize":18.0,"textAlignHorizontal":"LEFT","textAlignVertical":"CENTER","letterSpacing":-0.09,"letterSpacingValue":-0.5,"letterSpacingUnit":"PERCENT","lineHeightPx":26.1000003814697,"lineHeightPercent":115.079368591309,"lineHeightPercentFontSize":145.0,"lineHeightUnit":"FONT_SIZE_%","paragraphSpacing":0,"paragraphIndent":0,"listSpacing":0,"italic":false,"textCase":"ORIGINAL","textDecoration":"NONE","textDecorationSkipInk":false,"textDecorationStyle":"solid","textTruncation":"DISABLED"},"styleOverrideTable":{}},"47206:72090":{"type":"FRAME","id":"47206:72090","name":"Frame 54","absoluteBoundingBox":{"x":33778.0,"y":27609.0,"width":736.0,"height":211.440185546875},"isolatedAbsoluteRenderBounds":{"x":33777.328125,"y":27609.0,"width":736.671875,"height":212.783203125},"relativeTransform":[[1.0,0.0,32.0],[0.0,1.0,406.0]],"size":{"x":736.0,"y":211.440185546875},"fills":[],"strokeAlign":"INSIDE","layoutAlign":"STRETCH","strokes":[],"effects":[],"accessibleHTMLTag":"AUTO","isDecorativeImage":false,"ariaAttributes":{},"interactions":[],"layoutMode":"VERTICAL","itemSpacing":32.0,"counterAxisSizingMode":"FIXED","children":["47206:72091","47206:72097"]},"47206:74569":{"type":"TEXT","id":"47206:74569","name":"Örnekler Phenome Omics’e ulaştığında öncelikle bir kabul kontrolünden geçirilir ve belirlenen gereksinimleri karşılayıp karşılamadığı değerlendirilir. Bu aşamada kriterleri karşılamayan örnekler için sizinle iletişime geçilerek olası seçenekler paylaşılır. Kabul kontrolünü geçen örnekler için bilgilendirme yapılır ve lipid ekstraksiyonu ile analiz sürecine alınır. Laboratuvarımızda; kapsamlı lipidomik analizleri için örnek hazırlama, lipid tanımlama ve veri analizi süreçleri en son teknolojiye sahip sistemlerle yürütülmektedir. Temel hedefimiz, sadece birkaç mikrolitrelik örnek materyalinden mümkün olan en kapsamlı lipid bilgisini elde etmektir. Hedeflenmemiş (Untargeted) Lipidomik Analizleri için Thermo Scientific Orbitrap Exploris 240 MS sistemini kullanmaktayız. Bu ileri teknoloji sistem şu üstün özellikleri sunmaktadır:   Olağanüstü kütle doğruluğu 240.000’e varan ultra yüksek çözünürlük Düzenli ve hassas iyon yakalama m/z 40’a kadar genişletilmiş düşük kütle aralığı Dayanıklı ve yüksek cihaz performansı AcquireX akıllı veri toplama teknolojisi   Bu teknolojik altyapı sayesinde, özellikle düşük bollukta bulunan bileşenler için AcquireX Deep Scan yöntemini kullanarak en nadir moleküller bile saptanabiliyor. Kütle spektrometrisinden elde edilen lipidomik veriler, LipidSearch ve Compound Discoverer 3.3 yazılımları kullanılarak işlenir. Compound Discoverer yazılımının sunduğu gelişmiş araçlar sayesinde entegre veri tabanları ve spektral kütüphaneler ile özellik tespiti, veri sadeleştirme ve karmaşıklığın giderilmesi, bilinmeyen bileşenlerin tanımlanması, istatistiksel analizler ve metabolik ağ haritalama süreçleri başarıyla yürütmekteyiz.","absoluteBoundingBox":{"x":34626.0,"y":30811.12890625,"width":343.0,"height":1404.0},"isolatedAbsoluteRenderBounds":{"x":34626.0703125,"y":30814.748046875,"width":340.28125,"height":1372.16015625},"relativeTransform":[[1.0,0.0,0.0],[0.0,1.0,50.0]],"size":{"x":343.0,"y":1404.0},"fills":[{"opacity":0.550000011920929,"blendMode":"NORMAL","type":"SOLID","color":{"r":1.0,"g":1.0,"b":1.0,"a":1.0},"visible":true}],"strokeAlign":"OUTSIDE","layoutAlign":"STRETCH","strokes":[],"effects":[],"accessibleHTMLTag":"AUTO","isDecorativeImage":false,"ariaAttributes":{},"interactions":[],"characterStyleOverrides":[],"characters":"Örnekler Phenome Omics’e ulaştığında öncelikle bir kabul kontrolünden geçirilir ve belirlenen gereksinimleri karşılayıp karşılamadığı değerlendirilir.\n\nBu aşamada kriterleri karşılamayan örnekler için sizinle iletişime geçilerek olası seçenekler paylaşılır. Kabul kontrolünü geçen örnekler için bilgilendirme yapılır ve lipid ekstraksiyonu ile analiz sürecine alınır.\n\nLaboratuvarımızda; kapsamlı lipidomik analizleri için örnek hazırlama, lipid tanımlama ve veri analizi süreçleri en son teknolojiye sahip sistemlerle yürütülmektedir. 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