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Bu aşamayı geçemeyen örnekler için sizinle iletişime geçilerek olası seçenekler görüşülür. Kabul kontrolünü geçen örnekler, hazırlık ve analiz sürecine alınır. \n\nLaboratuvarımızda örnek hazırlama, metabolit tanımlama ve global metabolomik veri analizi süreçleri en son teknolojiye sahip sistemlerle yürütülmektedir. 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Bu aşamayı geçemeyen örnekler için sizinle iletişime geçilerek olası seçenekler görüşülür. Kabul kontrolünü geçen örnekler, hazırlık ve analiz sürecine alınır. Laboratuvarımızda örnek hazırlama, metabolit tanımlama ve global metabolomik veri analizi süreçleri en son teknolojiye sahip sistemlerle yürütülmektedir. Temel hedefimiz, sadece birkaç mikrolitrelik örnek materyalinden mümkün olan en kapsamlı metabolik bilgiyi elde etmektir. Hedeflenmemiş metabolomik analizlerde Thermo Scientific Orbitrap Exploris 240 MS sistemi kullanılmaktadır. Bu ileri teknoloji platformu, belirtilen teknik avantajları sunar:   Olağanüstü kütle doğruluğu 240.000’e varan ultra yüksek çözünürlük Düzenli ve hassas iyon yakalama m/z 40’a kadar genişletilmiş düşük kütle aralığı Dayanıklı ve yüksek cihaz performansı AcquireX akıllı veri toplama teknolojisi   Bu altyapı sayesinde, özellikle düşük bollukta bulunan bileşenler AcquireX Deep Scan yaklaşımı ile yüksek hassasiyetle tespit edilebilir. Kütle spektrometrisinden elde edilen metabolomik veriler, Compound Discoverer 3.3 yazılımı kullanılarak analiz edilir. 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Bu aşamayı geçemeyen örnekler için sizinle iletişime geçilerek olası seçenekler görüşülür. Kabul kontrolünü geçen örnekler, hazırlık ve analiz sürecine alınır. \n\nLaboratuvarımızda örnek hazırlama, metabolit tanımlama ve global metabolomik veri analizi süreçleri en son teknolojiye sahip sistemlerle yürütülmektedir. 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Literatür tabanlı protokollerle metabolomik analizlere uygun örnek hazırlığı UHPLC tabanlı ayırma sonrası LC-MS/MS Orbitrap teknolojisi ile ölçüm Ölçümlerin Thermo Scientific Orbitrap Exploris 240 MS sistemi ile gerçekleştirilmesi Metabolit tanımlama ve anotasyon için Compound Discoverer yazılımının kullanımı Verilerin uygun veri tabanları ve Compound Discoverer 3.3 aracılığıyla işlenmesi Analiz sonuçları yaklaşık 2 ila 3 hafta içinde teslim edilmektedir.","absoluteBoundingBox":{"x":30830.0,"y":28722.439453125,"width":736.0,"height":286.0},"isolatedAbsoluteRenderBounds":{"x":30830.341796875,"y":28729.478515625,"width":706.841796875,"height":276.740234375},"relativeTransform":[[1.0,0.0,0.0],[0.0,1.0,50.0]],"size":{"x":736.0,"y":286.0},"fills":[{"opacity":0.550000011920929,"blendMode":"NORMAL","type":"SOLID","color":{"r":1.0,"g":1.0,"b":1.0,"a":1.0},"visible":true}],"strokeAlign":"OUTSIDE","layoutAlign":"STRETCH","strokes":[],"effects":[],"accessibleHTMLTag":"AUTO","isDecorativeImage":false,"ariaAttributes":{},"interactions":[],"characterStyleOverrides":[],"characters":"LC-MS teknolojisi, bir karışım içindeki bileşenlerin önce UHPLC ile ayrıştırılmasını, ardından iyonlaştırılarak kütle/yük (m/z) oranlarına göre analiz edilmesini kapsar. 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Vücudumuzdaki binlerce metabolit; tükettiğimiz gıdalardan, mikrobiyotamızdan ve vücudumuzda süregelen sayısız kimyasal süreçten köken alır. Metabolit modellerindeki bozulmaların, organizmanın hayatta kalması üzerinde dramatik etkiler yaratabilen metabolik hastalıklara yol açtığı iyi bilinmektedir. Bu nedenle, günümüz klinik kimyasında metabolitlerin ölçümü hayati bir önem taşır. Metabolit profillerinin metabolik bozuklukların taranmasında (klinik kimya uygulamaları) kullanılmasının yanı sıra, temel biyolojik süreçleri anlamak için hedefli veya hedeflenmemiş profilleme çalışmaları gereklidir. Metabolitler, transkript verilerine kıyasla fenotipe daha yakın \"uç noktalar\" olarak kabul edildikleri için, doğrudan belirli fenotiplerle ilişkilendirilebilen biyokimyasal bilgiler sağlarlar. Bu bağlantılar; hedeflenen biyolojik süreçlerin keşfinde gen, protein veya diğer meta-verilerle ilişki kurmak ya da yeni biyobelirteçler tanımlamak amacıyla kullanılır. 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Bu sayede farmakodinamik, etkinlik ve yanıt biyobelirteçlerinin belirlenmesine ve temel biyolojik yol haritalarındaki değişimlerin açığa çıkarılmasına yardımcı olur.","absoluteBoundingBox":{"x":30830.0,"y":28052.439453125,"width":736.0,"height":572.0},"isolatedAbsoluteRenderBounds":{"x":30830.197265625,"y":28059.478515625,"width":734.57421875,"height":536.740234375},"relativeTransform":[[1.0,0.0,0.0],[0.0,1.0,132.0]],"size":{"x":736.0,"y":572.0},"fills":[{"opacity":0.550000011920929,"blendMode":"NORMAL","type":"SOLID","color":{"r":1.0,"g":1.0,"b":1.0,"a":1.0},"visible":true}],"strokeAlign":"OUTSIDE","layoutAlign":"STRETCH","strokes":[],"effects":[],"accessibleHTMLTag":"AUTO","isDecorativeImage":false,"ariaAttributes":{},"interactions":[],"characterStyleOverrides":[],"characters":"Metabolitler, tüm canlı organizmaların temel bileşenleridir; amino asitler, lipidler, organik asitler ve çevresel kimyasallar gibi oldukça çeşitli bir grubu temsil ederler. Vücudumuzdaki binlerce metabolit; tükettiğimiz gıdalardan, mikrobiyotamızdan ve vücudumuzda süregelen sayısız kimyasal süreçten köken alır. Metabolit modellerindeki bozulmaların, organizmanın hayatta kalması üzerinde dramatik etkiler yaratabilen metabolik hastalıklara yol açtığı iyi bilinmektedir.\n\nBu nedenle, günümüz klinik kimyasında metabolitlerin ölçümü hayati bir önem taşır. Metabolit profillerinin metabolik bozuklukların taranmasında (klinik kimya uygulamaları) kullanılmasının yanı sıra, temel biyolojik süreçleri anlamak için hedefli veya hedeflenmemiş profilleme çalışmaları gereklidir. Metabolitler, transkript verilerine kıyasla fenotipe daha yakın \"uç noktalar\" olarak kabul edildikleri için, doğrudan belirli fenotiplerle ilişkilendirilebilen biyokimyasal bilgiler sağlarlar. 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