Whole Transcriptome Sequencing
Total RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples
Service summary
- RNA extractions with QIAsymphony Paxgene Blood RNA Kit on QIAsymphony*
- QC check with Invitrogen Qubit, Agilent Bioanalyzer and Tapestation
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq 6000, ≥25 million reads (R1 + R2 = 50 M Reads) output per sample
- Turnover time approx. 4 to 5 weeks**
- (*) For unextracted samples.(**) Turnover times are not guaranteed, may change according to your project.
Sample requirements
Accepted Sample Type
Recommended Amount
Shipment Method
Whole Blood (Fresh or Frozen)
2.5 mL in PaxGene tube650μL -1mL in 2mL tubes
RT for ≤ 3 daysDry ice for ≥ 3 days
Tissue
30 - 50 mg
Dry ice
FFPE Tissue
- 4-6 slices for 10µm,
- 8-10 slices for 5µm
Room temprature
Cell Pellet
- ≥10^6 cells (eukaryotic)
- ≥10^8 cells (microorganism)
Dry ice
Total RNA
50μl, >30 ng/μLRIN ≥7.0
Dry ice
How to evaluate the sample quality
We check your samples upon arrival, however we still require our users to do their own QC steps before sending samples. To accomplish this we recommend using the following methods.
Concentration
Fluorometric measurements (Qubit, Quant-it). At least 100 ng RNA recommend input. The protocol supports a broad range of RNA inputs, from 1 ng to 1000 ng. The protocol is optimized for 10–100ng RNA input from low-quality RNA (RIN≥2) samples or FFPE (DV200>55%) samples. Library performance can vary with lower input amounts and lesser quality RNA. Do not use absorbance measurements (Nanodrop, spectrophotometer).
RNA quality
Capillary electrophoresis (Fragment Analyzer, Bioanalyzer, TapeStation), RIN ≥7.0. Do not use gel electrophoresis. For FFPE samples we recommend using capillary electrophoresis to determine the DV200 value.
If you are not able to carry out these steps, or your samples are below the required thresholds, please get in touch.
What we do with your samples
Once your samples arrive at Phenome Omics, we start by performing a reception control step in which we make sure the sample meets our requirements.
If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, Phenome Omics will only make one attempt at library preparation and if the prep does not work you will have to pay for the library preparation anyway.
If the samples pass reception control, the samples will be queued for library preparation.
Library preparation
Illumina’s Ribo-Zero Plus depletion kit supports ribosomal RNA (rRNA) depletion from human, mouse, rat, and bacterial species in addition to globin RNAs. The kit is also compatible with low input samples. Total RNA-sequencing with ribosomal depletion offers a comprehensive whole transcriptome analysis by capturing diverse RNA molecules (not small RNA) such as mRNAs, non-coding RNAs that are not polyadenylated and unspliced transcripts.
We evaluate the concentration of the libraries using qPCR and look at the fragment size distribution of the library molecules using Bioanalyzer. Once the libraries have passed this QC step, they are normalized, pooled, and queued for sequencing.
Science & Innovation
Awards
Omics Library
Language
English
Contact Us
Whole Transcriptome Sequencing
Total RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples
Service summary
- RNA extractions with QIAsymphony Paxgene Blood RNA Kit on QIAsymphony*
- QC check with Invitrogen Qubit, Agilent Bioanalyzer and Tapestation
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq 6000, ≥25 million reads (R1 + R2 = 50 M Reads) output per sample
- Turnover time approx. 4 to 5 weeks**
- (*) For unextracted samples.(**) Turnover times are not guaranteed, may change according to your project.
Sample requirements
Accepted Sample Type
Recommended Amount
Shipment Method
Whole Blood (Fresh or Frozen)
2.5 mL in PaxGene tube650μL -1mL in 2mL tubes
RT for ≤ 3 daysDry ice for ≥ 3 days
Tissue
30 - 50 mg
Dry ice
FFPE Tissue
- 4-6 slices for 10µm,
- 8-10 slices for 5µm
Room temprature
Cell Pellet
- ≥10^6 cells (eukaryotic)
- ≥10^8 cells (microorganism)
Dry ice
Total RNA
50μl, >30 ng/μLRIN ≥7.0
Dry ice
How to evaluate the sample quality
We check your samples upon arrival, however we still require our users to do their own QC steps before sending samples. To accomplish this we recommend using the following methods.
Concentration
Fluorometric measurements (Qubit, Quant-it). At least 100 ng RNA recommend input. The protocol supports a broad range of RNA inputs, from 1 ng to 1000 ng. The protocol is optimized for 10–100ng RNA input from low-quality RNA (RIN≥2) samples or FFPE (DV200>55%) samples. Library performance can vary with lower input amounts and lesser quality RNA. Do not use absorbance measurements (Nanodrop, spectrophotometer).
RNA quality
Capillary electrophoresis (Fragment Analyzer, Bioanalyzer, TapeStation), RIN ≥7.0. Do not use gel electrophoresis. For FFPE samples we recommend using capillary electrophoresis to determine the DV200 value.
If you are not able to carry out these steps, or your samples are below the required thresholds, please get in touch.
What we do with your samples
Once your samples arrive at Phenome Omics, we start by performing a reception control step in which we make sure the sample meets our requirements.
If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, Phenome Omics will only make one attempt at library preparation and if the prep does not work you will have to pay for the library preparation anyway.
If the samples pass reception control, the samples will be queued for library preparation.
Library preparation
Illumina’s Ribo-Zero Plus depletion kit supports ribosomal RNA (rRNA) depletion from human, mouse, rat, and bacterial species in addition to globin RNAs. The kit is also compatible with low input samples. Total RNA-sequencing with ribosomal depletion offers a comprehensive whole transcriptome analysis by capturing diverse RNA molecules (not small RNA) such as mRNAs, non-coding RNAs that are not polyadenylated and unspliced transcripts.
We evaluate the concentration of the libraries using qPCR and look at the fragment size distribution of the library molecules using Bioanalyzer. Once the libraries have passed this QC step, they are normalized, pooled, and queued for sequencing.
Science & Innovation
Awards
Omics Library
Language
English
Contact Us
Phenome Omics © 2026
Whole Transcriptome Sequencing
Total RNA sequencing based on reduced rRNA content and other type of highly abundant RNAs in both prokaryotic and eukaryotic samples
Service summary
- RNA extractions with QIAsymphony Paxgene Blood RNA Kit on QIAsymphony*
- QC check with Invitrogen Qubit, Agilent Bioanalyzer and Tapestation
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq 6000, ≥25 million reads (R1 + R2 = 50 M Reads) output per sample
- Turnover time approx. 4 to 5 weeks**
- (*) For unextracted samples.(**) Turnover times are not guaranteed, may change according to your project.
Sample requirements
Accepted Sample Type
Recommended Amount
Shipment Method
Whole Blood (Fresh or Frozen)
2.5 mL in PaxGene tube650μL -1mL in 2mL tubes
RT for ≤ 3 daysDry ice for ≥ 3 days
Tissue
30 - 50 mg
Dry ice
FFPE Tissue
- 4-6 slices for 10µm,
- 8-10 slices for 5µm
Room temprature
Cell Pellet
- ≥10^6 cells (eukaryotic)
- ≥10^8 cells (microorganism)
Dry ice
Total RNA
50μl, >30 ng/μLRIN ≥7.0
Dry ice
How to evaluate the sample quality
We check your samples upon arrival, however we still require our users to do their own QC steps before sending samples. To accomplish this we recommend using the following methods.
Concentration
Fluorometric measurements (Qubit, Quant-it). At least 100 ng RNA recommend input. The protocol supports a broad range of RNA inputs, from 1 ng to 1000 ng. The protocol is optimized for 10–100ng RNA input from low-quality RNA (RIN≥2) samples or FFPE (DV200>55%) samples. Library performance can vary with lower input amounts and lesser quality RNA. Do not use absorbance measurements (Nanodrop, spectrophotometer).
RNA quality
Capillary electrophoresis (Fragment Analyzer, Bioanalyzer, TapeStation), RIN ≥7.0. Do not use gel electrophoresis. For FFPE samples we recommend using capillary electrophoresis to determine the DV200 value.
If you are not able to carry out these steps, or your samples are below the required thresholds, please get in touch.
What we do with your samples
Once your samples arrive at Phenome Omics, we start by performing a reception control step in which we make sure the sample meets our requirements.
If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, Phenome Omics will only make one attempt at library preparation and if the prep does not work you will have to pay for the library preparation anyway.
If the samples pass reception control, the samples will be queued for library preparation.
Library preparation
Illumina’s Ribo-Zero Plus depletion kit supports ribosomal RNA (rRNA) depletion from human, mouse, rat, and bacterial species in addition to globin RNAs. The kit is also compatible with low input samples. Total RNA-sequencing with ribosomal depletion offers a comprehensive whole transcriptome analysis by capturing diverse RNA molecules (not small RNA) such as mRNAs, non-coding RNAs that are not polyadenylated and unspliced transcripts.
We evaluate the concentration of the libraries using qPCR and look at the fragment size distribution of the library molecules using Bioanalyzer. Once the libraries have passed this QC step, they are normalized, pooled, and queued for sequencing.
Science & Innovation
Awards
Omics Library
Language
English
Contact Us
Phenome Omics © 2026